Quantification of renal Na-K-ATPase activity by image analysing system
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چکیده
منابع مشابه
Regulation of renal proximal tubule Na-K-ATPase by prostaglandins.
Prostaglandins (PGs) play a number of roles in the kidney, including regulation of salt and water reabsorption. In this report, evidence was obtained for stimulatory effects of PGs on Na-K-ATPase in primary cultures of rabbit renal proximal tubule (RPT) cells. The results of our real-time PCR studies indicate that in primary RPTs the effects of PGE(2), the major renal PG, are mediated by four c...
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To determine the biochemical events of Na+ transport, we studied the interactions of Na+, Tris+, and K+ with the phosphorylated intermediates of Na,K-ATPase from ox brain. The enzyme was phosphorylated by incubation at 0 degrees C with 1 mM Mg2+, 25 microM [32P]ATP, and 20-600 mM Na+ with or without Tris+, and the dephosphorylation kinetics of [32P]EP were studied after addition of (1) 1 mM ATP...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1990
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990110802